Batman: Arkham Begins, that takes place after Origins.
Riddler: Batman meets Riddler face to face.
Scarecrow: Batman meets Scarecrow for the first time.
Clayface: Batman meets Clayface disguised as Harvey Dent.
Two Face: Harvey Dent turns into Two Face.
Poison Ivy: Pamala turns into a supervillain.
Scarface and Ventriloquist: Arnold Wesker created Scarface.
Victor Zsasz: Batman meets Victor for the first time.
Black Mask: Black Mask survived and now has a burned mask.
The Joker: Joker tries to take over Gotham again with a bomb.
Harley Quinn: Batman meet Harley for the first time.
Killer Moth: Batman meet Killer Moth.
Bane: Bane used more venom to crush Batman.
Batgirl: Barbara teams up with Batman for the first time.
KGBeast: Batman meet and face KGBeast for the first time.
Killer Croc: Croc escapes and now in a sewer after biting Aaron Cash's arm off.
Firefly: Batman faces Firefly at the a working building.
Mad Hatter: Batman faces Mad Hatter again.
Tweedledee and Tweedledum: Batman meet the Tweedle brothers for the first time.
Deathstroke: Working with Penguin to guard him.
Penguin: Working to take over Gotham.
Ratcatcher: Batman meets Ratctacher for the first time.
Great White Shark: Batman meets Great White Shark for the first time.
Mr. Freeze: Batman faces Mr. Freeze again.
Ra's Al Ghul: Batman meets The Demons Head for the first time.