This list includes all the Bosses that are fought in the Batman: Arkham game franchise.
Batman: Arkham Asylum
- Victor Zsasz (first encounter; Intensive Treatment)
- Titan Henchman (Intensive Treatment)
- Scarecrow (Medical Facility)
- Bane (Morgue)
- Scarecrow (Arkham Mansion)
- Harley Quinn (Penitentiary)
- Two Titan Henchmen (Botanical Gardens)
- Scarecrow (Intensive Treatment)
- Victor Zsasz (second encounter; Arkham Mansion)
- Killer Croc (Croc’s Lair)
- Titan Henchman (Sewers)
- Poison Ivy (Botanical Gardens)
- Two Titan-Infected Arkham Guards (Visitors Center)
- Titan Joker (Visitors Center rooftop)
Batman: Arkham City
Main Story
- Mr. Hammer (Steel Mill)
- The Penguin's Titan Henchman (Museum Training Area)
- Mr. Sickle (Museum War Room)
- The Penguin and Solomon Grundy (Iceberg Lounge)
- Ra's al Ghul (Wonder City)
- Mr. Freeze (GCPD Building)
- Fake Joker, Mr. Hammer, and The Joker's Titan Henchman (Steel Mill)
- Clayface (Monarch Theatre)
Side Missions
- Victor Zsasz (Zsasz’s hideout)
- The Mad Hatter (Mad Hatter’s Lair)
- Deadshot (mini-boss; Bowery rooftop)
- Riddler (mini-boss; Trainyard)
Catwoman Story Pack
- Poison Ivy (Poison Ivy’s Lair)
- Two-Face (Museum Armory)
Harley Quinn's Revenge
- Harley Quinn (Steel Mill)
- Harley's Titan Henchman (Steel Mill)
- Harley Quinn and Reprogrammed Mechanical Guardians (Steel Mill)
Batman: Arkham Origins
Main Story
- Killer Croc (Helipad)
- Electrocutioner (joke boss; The Final Offer)
- Deathstroke (The Final Offer)
- Copperhead (Steel Mill)
- Bane (Gotham Royal Hotel)
- Firefly (Pioneer’s Bridge)
- Venom Bane (Panopticon)
- TN-1 Bane (Maximum Security)
- The Joker (mini-boss) (Chapel)
Most Wanted Cases
- Anarky (Solomon Wayne Courthouse)
- Shiva (Wonder Tower)
- Deadshot (Gotham Merchant’s Bank)
- Black Mask (mini-boss) (Church)
- Bird (My Alibi)
Initiation
- Shiva (Dojo)
Cold, Cold Heart
- Mr. Freeze (GothCorp)
Batman: Arkham Origins Blackgate
- Catwoman (Gotham Rooftops)
- Bronze Tiger (Cell Blocks)
- Solomon Grundy (Industrial)
- Black Mask (Industrial)
- Deadshot (Administration)
- The Joker (Administration)
- The Penguin (Cell Blocks)
- Catwoman (Blackgate Lighthouse)
Batman: Arkham Knight
Main Story
- Militia Humvee (Gotham streets)
- The Arkham Knight's Attack Chopper (Ace Chemicals)
- Militia APC (Gotham streets)
- Christina Bell (mini-boss; Panessa Studios)
- Johnny Charisma (Panessa Studios)
- Albert King (Panessa Studios)
- The Cloudburst Tank (Gotham streets)
- The Arkham Knight's Excavator (Underground tunnels)
- The Arkham Knight (Militia HQ)
- The Joker (mini-boss) (Scarecrow Nightmare)
Most Wanted Cases
- Nine Militia APCs (Gotham streets)
- Two-Face (mini-boss) (Kingston Bank)
- Professor Pyg (Pretty Dolls Parlor)
- Firefly (Gotham streets)
- Deathstroke's Tank (Gotham streets)
- The Riddler (Pinkney Orphanage)
Harley Quinn Story Pack
- Nightwing (Blüdhaven Police Department)
Red Hood
- Black Mask (Sionis’ Hideout)
Scarecrow Nightmare
A Matter of Family
- The Joker and Harley Quinn (Seagate Amusement Park)
Iceberg Lounge DLC
- Killer Croc (Iceberg Lounge)
The Season of Infamy
- The Mad Hatter and Officer Hutch (GCPD Lockup)
- Killer Croc (Iron Heights Penitentiary)
Suicide Squad: Kill the Justice League
- The Flash Clone (Loomis Land)
- Green Lantern Clone (Power Plant)
- Batman Clone (Metropolis Batcave)
- Superman Clone (Centennial Park)
- Brainiacs #1 - #7 (Elseworlds)
- Brainiacs #11 - #13 (Elseworlds)
Batman: Arkham Shadow
- Dick Grayson (mini-boss) (Monarch Theatre)
- Shrike (Solomon Wayne Courthouse)
- Carmine Falcone (Maximum Security)
- Lyle Bolton (Administration)
- Jonathan Crane and Shadow Batman (Arkham Wing)
- Two-Face (USS Halsey)