Arkham Origins Incident
Several of Bane's men in Gotham City used TN-1, to increase their muscles, strength, adrenaline, rage, tolerance, durability, stamina & metabolism, with Bane himself proposing to use it as an attempt to cure himself of his addiction to Venom and give him a permanent transformation into a true super-soldier. Also, according to Batman, Bane was aware of the memory loss and intellectual degeneration side effects as he was trying to find a way to avoid or remove these side effects. Bane finally (albeit reluctantly due to the side effects) injected himself with the serum in his final battle with Batman, grew to gargantuan proportions, and gained Meta-human strength, durability, stamina, adrenaline, aggression and metabolism, but also suffered memory loss, and lost his knowledge of Batman's true identity in the process.
Arkham Asylum Incident
The TN-1 in Bane's blood, coupled with his Venom and Poison Ivy's Plants, was used by Dr. Penelope Young in the creation of her Titan serum. Like the TN-1, Titan could turn any user into a Bane-like monster, including grotesque facial and muscular disfigurement; however, only a small amount was needed to trigger a long lasting transformation. The treatment had a price, as the sudden release of endorphin and adrenaline, along with the extreme pain of the transformation, caused the recipient to go into a crazed, and psychotic state. Only Bane (due to his extreme intelligence and will, and his previous usage of Venom and the TN-1), Poison Ivy (due to her natural immunity to toxins), and the Joker (because of his high intelligence and his psychopathy) retained their personalities (at least, partially) and some of their intelligence.
Arkham City Incident
Bane (while still under the influence of the Titan Formula) appeared notably more intelligent than his appearance in Arkham Asylum as he not only spoke in full sentences, but he was also much calmer and cunning in nature as demonstrated in his attempt to manipulate Batman (albeit unsuccessfully) in order to achieve his own goals. Bane's intelligence, however, was still a far cry from what it once was, as it seemed to be affected by his emotional state and the anxiety produced by his addiction to the drug, as he was unaware that Batman knew that he was going to try and keep the Titan for himself and used him to gather the rest for him. It eventually reached the point where lowly henchmen were able to manipulate Bane with the false hint of locations of Titan due to his emotional instability and addiction.
- TN-1 seemed to be an acronym that was close to that of "Titan", as a precursor to its creation.
- Despite claiming in an audio diary that a storage tank was no longer needed, Bane retained one in both of his appearances in Arkham Asylum and Arkham City, however, given the fact that a large amount of his blood was taken by Dr. Young, it was possible that he needed the Venom to sustain himself.
- Project TN-1 was the Arkhamverse's answer to a Super-Soldier Program. The other was the Military Serum Project of World War II, which infused Deathstroke with enhanced strength, speed, stamina, intelligence, and a vast healing factor.
- Shortly after his overdose, Bane forcefully regurgitated some of the Venom that was already in his body, suggesting that TN-1 may trigger a strong chemical reaction with the former when it comes into contact with it.